Accu Test HCV Chromatographic Immunoassay

Short Description

AccuTest HCV is a rapid chromatographic immunoassay for the qualitative detection of antibodies generated against proteins encoded by the conserved sequence of core NS3, NS4, and NS5 parts of the HCV genome in human whole blood/serum or plasma.

Dosage Form

Lateral flow chromatographic immunoassay

Introduction

Hepatitis C Virus (HCV) is a small, enveloped, positive-sense, single-stranded RNA virus. Antibody to HCV is found in over 80% of individuals with well-documented non-A, non-B hepatitis. Conventional methods fail to isolate the virus in cell culture or visualise it by electron microscope. Cloning the viral genome has allowed it to develop serologic assays that use recombinant antigens. New serologic tests have added multiple antigens using recombinant protein and/or synthetic peptides to avoid nonspecific cross-reactivity and increase HCV antibody test sensitivity.

Allow the test, specimen and or control to room temperature before the testing. Remove one test card from the pouch and place it on a clean flat surface. Using the dropper, provide one drop of serum/plasma or W. blood sample (30ul) then two drops of buffer (40ul) immediately into the sample well. Avoid overflowing. Read results within 15 minutes.

The membrane is coated with recombinant HCV antigen on the test line region of the device. During testing, the serum/plasma or w.blood specimen reacts with HCV antigen-coated particles. The mixture migrates upward on the membrane chromatographically by capillary action to react with recombinant HCV antigen or membrane and generate a coloured line.